Electrochemical DNA Biosensor For The Detection Of Sanguinarine In Adulterated Mustard Oil
Ravindra P. Singh, Da-Yeon Kang, Jeong-Woo Choi
Volume 1, Issue 1, Page 48-54 | DOI: 10.5185/amlett.2010.3106
Double stranded deoxyribonucleic acid; polyaniline; biosensor; sanguinarine
PANi/ClO4 doped films were developed electrochemically to immobilize DNA as biosensing platform to detect sanguinarine from adulterated mustard oils. The principle of technique was based on the interaction/intercalation of sanguinarine with dsDNA using electrochemical method. Further, it was suggested that sanguinarine intercalates with DNA strands forming complexes, results in the decrease of redox peak currents. In addition, the decrease of the peak current is proportional to the concentration of sanguinarine. The results based on the voltammetric signals decreased in concomitant increase of sanguinarine concentrations due to base pairing in dsDNA. The bioelectrode exhibited the detection limits 2–64 µM. The recovery experiment results found between 89% and 121% from spiked edible mustard oil sources. The correlation found between the current vs. concentration of SA with a correlation coefficient of (r2) of 0.995 at 95% confidence limit. UV–VIS, CV, DPV, AFM, and SEM. characterized the bioelectrodes. Thus, the proposed electrochemical DNA biosensor detected SA and promising for real-time analysis of small molecules of environmental interest. Copyright © 2010 VBRI press.