Studies On The Interaction Of DNA With Vitamin B12 Based On The Immobilization Of DsDNA On Nano-scale Hydroxyapatite Coating
Nuo Zhang, Caixia Xu, Qin Wei, Bin Du, Ru Li, Tianguo Zhang, Dan Wu, Yuxue Dai
Volume 1, Issue 1, Page 34-39 | DOI: 10.5185/amlett.2010.3104
Vitamin B12; DNA; hydroxyapatite; interaction; cyclic voltammetry
Nano-scale hydroxyapatite (HAp) was prepared by combining co-precipitation with microemulsion method, which exhibited strong adsorption for DNA due to its excellent biocompatibility and particular adsorbability. DNA and HAp could be modified onto glassy carbon electrode (GCE) by the simple and convenient “tip-coating” method. Cyclic voltammetry was used to investigate the interaction of DNA immobilized on the HAp film with vitamin B12 (VB12). The existence of DNA led to the decrease of reduction current of VB12. Both the electron transfer coefficient (α) and the standard rate constant (ks) were different obtained on GCE and dsDNA/HAp/GCE, which indicated the formation of an electrochemical inactive super molecular complex DNA-nVB12. The equilibrium constant of this complex was calculated to be 5.35 × 105 mol·L-1 and the binding number between DNA and VB12 of the complex were determined to be one. Copyright © 2010 VBRI press.