A High Throughput Chemiluminescence Method Based On Molecularly Imprinted Sol-gel Films For Determination Of Sibutramine
Fuwei Wan, Jinghua Yu, Shenguang Ge, Mei Yan, Kexiao Yu, Meng Zhang
Volume 1, Issue 2, Page 164-169 | DOI: 10.5185/amlett.2010.7138
Sibutramine; chemiluminescence; molecularly imprinted film; high throughput
A novel and high throughput chemiluminescence (CL) method for determination of sibutramine was originally developed based on potassium permanganate-hydrochloric acid-(tween-80) CL system. Molecularly imprinted sol-gel film (MIF) was synthesized and used as selective material to improve the selectivity of CL. In the proposed procedure, sibutramine molecule was adsorbed into the MIF, and then potassium permanganate, hydrochloric acid and tween-80 were added in wells of a 96-well plate, Based on which, sibutramine was oxidized and CL was produced and detected by a photomultiplier tube (PMT). The CL intensity was correlated linear with the concentration of sibutramine over the range of 5.0 × 10-8-1.0 × 10-6 g×mL-1 and the detection limit was 1.8 × 10-8 g×mL-1. The relative standard deviation (RSD) was 3.1% for determination of 1.0 × 10-7 g×mL-1 sibutramine (n = 11). The fabricated molecularly imprinted sol-gel film possessed good selectivity and could be regenerated. This method was successfully applied to the determination of sibutramine in weight-reducing tonic. Copyright © 2010 VBRI press.