Indian materials smart materials
News Insight

Advanced Materials World Congress will be held in Stockholm, Sweden during 23-26 August 2015.

Online Advertising

Adv Mat Lett is an official journal of IAAM

VBRI Press is a member of CrossRefDOAJ
Adv Mat Lett is a member of DOAJ

fullabstractpdf

Get PDF

Full Article abstractpdf

Get PDF

Voltammetric Detection Of Microcystis Genus Specific-sequence With Disposable Screenprinted Electrode Modified With Gold Nanoparticles

Minbo Lan, Chen Chen, Qin Zhou, Yuanjie Teng, Hongli Zhao, Xiangheng Niu

Volume 1, Issue 3, Page 217-224, Year 2010 | DOI: 10.5185/amlett.2010.

Keywords: Microcystis; water bloom; gold nanoparticles; screen printed carbon electrode

Abstract: 

A disposable electrochemical biosensor for detection of DNA sequence related to Microcystis was described. After depositing gold nanoparticles (AuNPs) onto a screen-printed carbon electrode (SPCE) surface by electrodeposition, the DNA probe which is complementary to a specific gene sequence related to Microcystis was immobilized onto the AuNPs modified SPCE via a thiol linker at the 5’ end. On the electrode surface the immobilized single-stranded DNA could undergo hybridization in the solution containing DNA specific-sequence of Microcystis. Using methylene blue as the indicator, DNA immobilization and hybridization were characterized by cyclic voltammetry and differential pulse voltammetry. Parameters, such as the deposition conditions of gold nanopariticle and the preconcentration time of MB, were optimized. In addition, the control experiments with non-complementary and single base mismatch sequences were investigated and results demonstrated the high selectivity of the biosensor. Copyright © 2010 VBRI press.

fullabstractpdf

Get PDF

Full Article abstractpdf

Get PDF

Full Article